FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006127339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255295940
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTAT381909391.1731940721801164TruSeq Adapter, Index 8 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTATGCCGT169753520.5214687792717243TruSeq Adapter, Index 8 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)