FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006127341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836226805
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTAT2452883348.648403349392927TruSeq Adapter, Index 8 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTATGCCGT282810450.9971362286733624TruSeq Adapter, Index 8 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTATGCC139823710.4929919911676457TruSeq Adapter, Index 8 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCGCGTAT31856810.11232109485686918TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCACGTAT30831430.10870579865350366TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCCCGTAT30020160.10584541386844415TruSeq Adapter, Index 8 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)