FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006127343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441855952
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTAT4833864910.93991124962825TruSeq Adapter, Index 27 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTATGCC23045780.5215677167114409TruSeq Adapter, Index 27 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTAT8734800.19768433491646165TruSeq Adapter, Index 1 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTATGCCGT8603300.19470825188748392TruSeq Adapter, Index 27 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTATG6175160.13975504849598586TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCGCGTAT5593480.12659057719335645TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)