FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006127351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570608723
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT6540399511.462144261681747TruSeq Adapter, Index 15 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATGCC45471050.7968866960346136TruSeq Adapter, Index 15 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATGCCGT41774760.7321086817665071TruSeq Adapter, Index 15 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATG13399470.2348276403759078TruSeq Adapter, Index 15 (97% over 36bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT12024470.21073056746803362TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCATCTCGTATG5987880.10493845885352861TruSeq Adapter, Index 15 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)