FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006127353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913772434
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTAT673324057.368618541626963TruSeq Adapter, Index 1 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCC74577730.8161521099245592TruSeq Adapter, Index 1 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCCGT46863990.5128628119679084TruSeq Adapter, Index 1 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATG12318950.1348142003592111TruSeq Adapter, Index 1 (97% over 36bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTAT10995190.12032744248881555TruSeq Adapter, Index 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)