FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006127359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657038632
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC7418095011.290196099154182TruSeq Adapter, Index 3 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCT39726230.6046254826611169TruSeq Adapter, Index 3 (100% over 50bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC19956580.3037352604252957TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC8869130.13498643105661404TruSeq Adapter, Index 3 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)