FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006127361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702516563
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTAT1550194965.736116408031058TruSeq Adapter, Index 5 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTATGCC141995600.5254199065569242TruSeq Adapter, Index 5 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTATGCCGT94564720.34991356313844724TruSeq Adapter, Index 5 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTATG56009630.20724990465118567TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)