FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006127363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121456665
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT613905411.9667273195990371TruSeq Adapter, Index 5 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTATGCC66953810.21449540130008501TruSeq Adapter, Index 5 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTATGCCGT40406600.12944789672420456TruSeq Adapter, Index 5 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)