FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006127365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432727536
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT437315181.2739583186074341TruSeq Adapter, Index 20 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATGCCGT53662990.1563275542180986TruSeq Adapter, Index 20 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTATGCC42868230.12488095705362151TruSeq Adapter, Index 20 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)