FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37155387
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCC156.883756E-494.0692394
ACGCTCT750.082.051081
CGCTCTT800.076.379812
TCTTCCG950.074.1965265
GCTCTTC1100.064.086413
CTCTTCC1100.064.084774
CTTCCGA1100.064.078826
CCGATCT1150.061.2935269
TCCGATC1200.058.7389228
ATGCTCT250.005091242556.8045921
TTCCGAT2050.038.9682587
TAGCTCT250.00170265437.6003286-87
AACCCAA300.00416400531.33128274-75
AAACCCA300.00416400531.33128274-75
CGATCTT1352.6073394E-819.14476810-11
AACTTCA650.00804380918.07684592-93
CTCCAGT11300.010.822124594
GCACACG38300.010.8069194
CTGTTAA3750.002726574210.0985941
GAGCACA48450.09.80494694
Produced by FastQC (version 0.11.5)