FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37155387
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGGA600.004162490431.333574
CGGAAGG1602.1191227E-929.39948394
GTTGGGG1659.088035E-825.7072281
TGGGGAG1551.541599E-624.2713182
GCGTCGG1100.003046569421.38144394
TTTTGGG1150.003948427320.4459482
TTGGGGG2201.4829893E-619.2377782
GTGGGGA1751.05626896E-418.8519671
ATATCGG1300.00800500318.0919994
GGAAGAA2658.720866E-615.97556894
GTGGGGG7800.015.7099711
TGGGGGG8100.015.6752252
CGACGGG1000.00566480514.10052390-91
GACTCAG1000.0056704214.09841672-73
AGAGCGG1504.477706E-412.53353788-89
AAGAGAG2750.00330321711.97360894
TAGATCG35550.011.2470694
AGATCGG129000.010.64762794
CTTCGGC1550.00811368510.61331886-87
TGTAGGG20550.010.07164794
Produced by FastQC (version 0.11.5)