FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31199641
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG1400.093.9798055
CTTCCGA1450.090.739126
ACGCTCT1400.087.9741441
TCCGATC1550.084.884988
GCTCTTC1600.082.243133
CCGATCT1600.082.232859
CGCTCTT1550.078.8466642
CTCTTCC1950.067.4808964
TTCCGAT2900.046.9899027
CGATCTG851.2369128E-1030.40542410-11
CGATCTA404.7896145E-429.36887410-11
TTCACTT350.00884185126.85571988-89
CACTTTT350.00884216226.85552690-91
CGATCTT1751.5188562E-917.45350310-11
CTGCTTG1901.8932022E-514.8552794
TCTTCTG2258.410647E-611.48848252-53
ACACGTC27450.010.79645394
GAGCACA42750.010.56374894
TCTGCTT2452.1626622E-510.55003756-57
TTCTGCT2351.6471266E-49.99942254-55
Produced by FastQC (version 0.11.5)