FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24692195
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT353.619789E-1093.9514549
CTTCCGA401.0404619E-982.214026
TCTTCCG401.0440999E-982.182215
ACGCTCT301.3658682E-679.189171
CGCTCTT353.5791618E-667.3361742
TCCGATC506.0754246E-965.786018
GCTCTTC452.2155655E-762.6235163
TTCCGAT802.4697692E-741.107017
CTCTTCC851.765481E-533.151014
CATGAAT2150.00850983313.25958251
GAGCACA27450.012.323694
TCCAGTC5656.469418E-811.64198594
AACTCCA7101.035005E-911.24962494
TGAACTC9000.010.44082894
AGAGCAC30850.010.35621894
ACACGTC17450.010.23141394
CACGTCT15500.010.00298694
CTGAACT9653.6379788E-129.7375694
AAGAGCA37600.09.62166794
ACTCCAG6450.09.46276192-93
Produced by FastQC (version 0.11.5)