FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24692195
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAGG700.00889155726.8247994
AGAGCGG1150.00397350120.42357694
GAGATCG2052.0139305E-713.7403892-93
CGGAAGA49250.011.25473894
TGGTCGC3903.3109344E-410.84020694
GAGCGTC27850.010.79479694
CCAGATC10900.010.34294994
AAGAGCG35750.010.24892294
CGTGTAG15550.09.96880794
GTCGTGT18700.09.79676394
ATCGGAA63600.09.74936894
AAGATCG42500.09.72642994
CCTCAGT2209.142243E-49.70807978-79
AGATCGG76400.09.6530894
TCGGAAG55200.09.53100394
CAGATCG23250.09.49586694
TAGCACT2502.77986E-49.49277280-81
TCAGATC9150.09.49187592-93
GTGGGGG4050.00551715179.272773
GAAGAGC39200.09.2270894
Produced by FastQC (version 0.11.5)