FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26986933
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT6000.090.110381
TCTTCCG5900.089.925135
CTTCCGA5900.089.129176
CGCTCTT6150.087.781892
CCGATCT6050.087.684869
CTCTTCC6000.087.653774
TCCGATC6000.087.633138
GCTCTTC6250.084.8666843
TTCCGAT7400.072.9571157
CGATCTT3800.037.66848410-11
CGATCTA2200.037.33165410-11
CGATCTG1300.034.29589510-11
GATCTAA2300.018.36439110-11
CAATAAG1550.001042418618.171962
ACAATAA1650.00148023617.2460061
CAAATAG2150.0092149913.1007172
ACAGTCA6954.7293724E-1112.28257594
TCTGCTT2105.3495853E-511.17251758-59
CTGCTTG1902.628849E-411.11371558-59
ACAGAAA3050.00672607510.8847731
Produced by FastQC (version 0.11.5)