FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26986933
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGGC300.00416894231.3236818-19
TGTCCGG300.0041801731.3064766-67
CATTCGG2355.2732503E-921.99738394
GAAGAGG1456.6104543E-419.44596594
AAGAGCA2600.002184699812.65234194
GAGATCG2350.00183879511.99857294
GAGCGTA1550.0081653210.60423888-89
GGAAGAG79750.010.48903694
CAGATCG37600.010.37376694
CAGAAGA2106.5728003E-410.06344792-93
GTAAAAG2351.659377E-49.99207592-93
CCAGATC17050.09.64694694
GATCGGA120600.09.58592494
CGTCGTG37850.09.56028994
TCGGAAG97300.09.51479894
CGGAAGA88150.09.43620194
GTAGGGA20650.09.33060994
TCGTGTA31000.09.24728894
TTAGATC15900.09.16243294
AAGAGTG12850.09.14288194
Produced by FastQC (version 0.11.5)