FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22259020
Sequences flagged as poor quality0
Sequence length100
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC4300.091.79328
CCGATCT4350.090.710379
TCTTCCG4300.090.647235
ACGCTCT4300.090.6427151
CGCTCTT4350.088.858982
CTTCCGA4400.088.618346
GCTCTTC4550.086.7141343
CTCTTCC4650.083.828664
TTCCGAT4950.081.6190267
CGATCTG1050.042.85752510-11
CGATCTT2850.037.3964510-11
CGATCTA1250.036.00031710-11
ACTCATG300.004182412831.30302876-77
CTGCTTG850.001932109216.5728656-57
TCTTCTG950.00405411214.82923352-53
GATCTAA1553.59422E-513.75224310-11
GTCTTCT1350.002916073412.17461652-53
CACACGT16400.011.74583994
GATCTAG2104.85983E-511.278295510-11
GATCTGG1800.001931688910.52640810-11
Produced by FastQC (version 0.11.5)