FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22259020
Sequences flagged as poor quality0
Sequence length100
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGG752.6375346E-950.1024994
GGGCAAA300.004185809831.29783428-29
CACGGTC350.0088550826.84747184-85
TAGCGAT551.0268993E-425.60792426-27
AGTTCCG550.002891670221.57464482-83
CGTGCTC1100.00307223721.3504994
GAGATCG1456.632537E-419.43630894
CATGCGA800.001198332817.79837480-81
CGACGAG850.001937946216.56538490-91
GATCGGA56200.011.95171694
TCGGAAG44050.011.83607494
TTAGATC9450.011.43211594
CAACGAG1450.004775487411.39720776-77
CAGTCAT1550.00754729610.71729980-81
ATCATTG2452.0532254E-510.59973576-77
CGTCGTG17200.010.37733294
ACAGTCA2807.5786957E-610.17049980-81
TAGATCG19800.09.96356394
CGGAAGA38600.09.856626594
AAGATCG32300.09.59256592-93
Produced by FastQC (version 0.11.5)