FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26148994
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC2700.092.1507348
CTTCCGA2700.090.423986
TCTTCCG2700.090.423985
CCGATCT2800.088.859639
ACGCTCT2650.085.8734051
CGCTCTT2700.083.454392
GCTCTTC2900.082.5508353
CTCTTCC3100.075.736264
TTCCGAT3600.069.1130457
ATGCTCT250.005060643456.891131
CACTATT205.716998E-446.93654364-65
CGATCTA1000.032.85051710-11
CGATCTG951.4551915E-1129.63956610-11
CGATCTT2400.026.3977410-11
CTGCTTG1206.8319714E-515.64135558-59
GTTAACT2802.5251793E-413.4127748
TTCTGCT2351.6731971E-412.10999394
GAGCACA37250.011.077894
AGAGCAC41550.011.07289694
TATAACA3500.001646542110.730228
Produced by FastQC (version 0.11.5)