FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27033980
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGAA300.004191568631.28905390-91
TTTGGTG650.0061145528.9636691
CGGAAGG1005.3544223E-528.17946294
GAGTTGA350.00889217326.82447692-93
GCGTCGG1050.00234675722.36465394
GCTAGAT850.001938415716.56479390-91
TTGGGGG2000.005613630614.1197891
GGAAAAG2204.7585854E-712.8025992-93
AGCGCGT1300.002202845412.63845492-93
TAGATCG31450.012.39478394
GATCGGA93850.011.45994994
GAAGAGC51200.010.91587394
CGTGTAG18200.010.83825594
AGGGGGG3100.00815979310.60517494
GCGTCGT29350.010.56129894
TCGGAAG72500.010.55920194
CGGAAGA64600.010.54185394
ATCGGAA83750.010.37452894
GTCGTGT21900.010.29386894
AGATCGG106100.010.00401994
Produced by FastQC (version 0.11.5)