FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25601270
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG1450.038.8696494
GTAGGGG1205.8811074E-627.39769694
CGGAAGG1301.0948674E-525.29018294
GGCCGCA550.002886375621.58146782-83
GATAGGA1605.2712538E-520.54827394
AAGGCAT600.005101855419.56018626-27
GGAAAGG1250.00644049418.78699194
CTGCCGC800.001195922517.80372680-81
AAGATAG2203.2178927E-517.07908494
AGCGCTC1054.020661E-415.82640982-83
AGATAGG1804.100184E-815.64804392-93
GATCGGC1502.8731549E-514.0832492-93
TCACGGC1100.00995877312.94756278-79
GAAGAGG1709.299863E-512.42638692-93
GTGTAGG27800.011.15055394
TCGGTGG5551.8102946E-711.00139294
GAGCGTC59050.010.97631294
TCAGAAG2208.5653475E-510.66697390-91
GATCAGA2502.7355365E-510.32770892-93
CAGTTAT2301.1962693E-410.32180-81
Produced by FastQC (version 0.11.5)