FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34016640
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT1750.089.39821
CTTCCGA1900.088.959026
TCCGATC2000.086.8476268
CTCTTCC1950.086.6850364
GCTCTTC1950.086.652413
CCGATCT2050.084.729279
TCTTCCG2000.084.511335
CGCTCTT1950.081.8434452
TTCCGAT3250.056.3335957
AGCCGAC352.954009E-453.6578256
GCCGACG405.712096E-446.9446647
TCCCCCC459.721131E-442.1405681
CCGACGT500.001712592237.555738
TAGCCGA600.00418405831.3004915
TGTAGCC600.00419017531.2911473
GAGCTCT300.00419598131.28234188-89
ACCTGCA350.00891005526.81343588-89
CGATCTT2100.025.70108210-11
CGATCTA601.8576065E-423.46620610-11
CGATCTG1006.0568163E-721.11958510-11
Produced by FastQC (version 0.11.5)