FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27807554
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT1150.085.722099
TCTTCCG1150.081.650375
CTTCCGA1150.081.650226
TCCGATC1200.078.238418
ACGCTCT1400.071.125971
GCTCTTC1350.069.5352553
CGCTCTT1400.067.0593262
CTCTTCC1700.058.0022474
TTCCGAT2150.043.667957
TTCTCTA459.343691E-426.19645990-91
TCCAGTT550.003090259221.3290472-73
CGATCTA705.266804E-420.1132210-11
CGATCTT1651.4084435E-817.06576210-11
CACATGT1300.00212993912.69520890-91
AAAAACA5102.1807136E-711.9658768
CACACGT36200.011.65778594
CTCTTTT1659.311299E-411.48782192-93
GCACATG1450.00482358411.3819190-91
TAAACAA2950.00566453211.1390299
TTAAACA4256.0101178E-511.04542358
Produced by FastQC (version 0.11.5)