FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24235864
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC804.7847605E-429.37387394
CGGAAGG650.006157727428.92196894
GATGACC350.00887173526.85611394
ATCAGGA350.00887191426.83701984-85
CCGGAAG650.00808070518.06248586-87
ATCGGCA1250.001634860613.14965192-93
GAAGAGC76150.012.65217494
AAGAGCG66450.012.30652594
CGTCGTG35100.012.18471894
CAGAAGA1751.2144349E-412.0762192-93
GTCGTGT30150.011.535212594
CAGATCG28550.010.86473294
TCGTGTA26450.010.83890394
GAGCGTC49100.010.72056694
GGAAGAG83600.010.70150692-93
CGGAAGA93950.010.55520994
GATCGGA132250.010.37691894
AGCGTCG43700.010.32454794
AAGATCG87500.010.25903594
AGAGCGT55500.010.24645394
Produced by FastQC (version 0.11.5)