FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26119592
Sequences flagged as poor quality0
Sequence length100
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGGG250.00525023156.36489594
TGTTGTG600.00413200731.3806291
TGGGGTT1005.3735093E-528.1647476
CGGAAGG1205.8779387E-627.39960194
AGACCGG1252.4356328E-422.54595894
ACTTCGG1050.00234531122.36702294
GTCGGGT650.00809798418.05579492-93
GTCATGT800.001289697517.604492-93
GCAAAGA2104.363732E-415.65691694
CCGCGGC2100.0078588713.42021494
GGAAGAG53700.012.68297794
GAGCGTC31750.012.27896694
CATGTCC2002.8956212E-511.86491480-81
AGAGCGT35900.011.513720594
ATCCAGC3101.6530976E-811.48174278-79
TTGTACT2156.056461E-511.0371380-81
TCGTGTA18400.010.97685994
GATCGGA85200.010.97086794
TCGGAAG67650.010.97025694
CATGTCA1550.007550478410.71669780-81
Produced by FastQC (version 0.11.5)