FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29720744
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCT2300.084.5477751
TCTTCCG2250.083.469985
CTTCCGA2200.083.232576
CCGATCT2100.080.477269
GCTCTTC2450.078.548313
CGCTCTT2500.076.984462
TCCGATC2300.075.5203258
CTCTTCC2800.065.403954
AAACTCG250.00526501356.3248833
TTCCGAT3800.049.4158557
CGATCTG701.9212166E-830.1707210-11
CGATCTT1850.027.9056510-11
CGATCTA605.9157064E-627.37713410-11
TCTGCTT2702.5120244E-913.03478956-57
GCCGTCT2951.5085583E-412.85838494
GTCTTCT2703.5111952E-812.16528152-53
CAGATCA6453.0009687E-811.02681294
TATAACA3450.001460298110.8858138
CACACGT37550.010.85942794
TTCTGCT2603.5835455E-610.82870254-55
Produced by FastQC (version 0.11.5)