FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006127789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006127789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29720744
Sequences flagged as poor quality0
Sequence length100
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGAGC450.001015029541.77220594
ATGGGGT450.001018271541.745142
GCAGGCG350.00887775426.83340870-71
GCGTCGG1101.0200957E-425.63294494
GATCGGG1150.003963978520.43205894
CGGAAGG1405.219404E-420.14017194
GTGGATG1200.005092562619.5663227
CCTGAGT758.344373E-418.78414292-93
GAGATCG4201.0059011E-915.664577594
GTGGTGG3001.655606E-615.6526614
GAACGGA2751.2503804E-515.37976694
TGGGTGG1900.00403459914.8399768
GATCAGA1859.4744064E-713.96118892-93
GGTGGGT2050.006717748513.7422746
AGAACGG2305.6512363E-813.27140692-93
TGGGGTG2500.00163607613.1483253
GTGGGTG2150.00919567913.1048857
TAGATCG41300.011.83377394
GGGGTGG7251.200533E-1011.6585354
AGATAGG1450.004973591311.335258592-93
Produced by FastQC (version 0.11.5)