FastQCFastQC Report
Wed 8 May 2024
EGAF00006128356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398743
Total Bases56.3 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT142953.585015912505047TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24790.6217037038894727No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA15000.37618215241396086No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT6260.15699335160742633No Hit
TGTCAAGTGGGAAATGAATGCTCTTGCAAGGCTCAAACTTGTGAACACAT6170.15473625869294258No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC5780.1449555227301796No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA5180.12990823663362117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)