FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291369
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT148785.1062398539309255TruSeq Adapter, Index 11 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12630.4334709595049576No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT5190.1781246460673579TruSeq Adapter, Index 11 (97% over 38bp)
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4360.149638430993002No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4030.13831258644536654No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA3680.12630032707666225No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA3150.1081103343183386No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA3120.10708071208673536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)