FastQCFastQC Report
Wed 8 May 2024
EGAF00006128360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319883
Total Bases46.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT115903.6231997324021594TruSeq Adapter, Index 9 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA14030.4385978623434193No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8650.270411369156848No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT5890.18412982246633927No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT4890.15286839250601TruSeq Adapter, Index 9 (97% over 36bp)
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC4870.15224316390680342No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4470.1397385919226717No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4330.13536199172822563No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGGAT3570.1116033049583754TruSeq Adapter, Index 9 (97% over 36bp)
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT3210.1003491901726569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)