FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319883
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT115893.6228871181025566TruSeq Adapter, Index 9 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA13890.43422126214897316No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8650.270411369156848No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT5850.1828793652679261No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT4890.15286839250601TruSeq Adapter, Index 9 (97% over 36bp)
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC4850.15161793530759682No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4440.13880074902386186No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4270.13348630593060587No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGGAT3570.1116033049583754TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)