FastQCFastQC Report
Wed 8 May 2024
EGAF00006128364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339333
Total Bases48.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT122823.619453457223435TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12690.37396893317184005No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA7280.21453852115768285No Hit
GCATTTCCTTGCTTGCTCACTTCAGTCCCTCTAGCCTTCTTTCTGTCCCT3690.1087427394329464No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATATCGTAT3560.10491169441227349TruSeq Adapter, Index 7 (97% over 36bp)
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3480.10255412824570553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)