FastQCFastQC Report
Wed 8 May 2024
EGAF00006128366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300555
Total Bases42.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT133674.4474389046929845TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12200.40591572257989383No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG5010.16669162050207117No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3740.12443645921711499No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3630.1207765633577881No Hit
GCGTGACAGCCACTGTAGGACTTTGATCTCAGGGGGACAAGCTGACACAG3130.10414067308812032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)