FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300555
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT133564.4437790088336575TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12200.40591572257989383No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG4940.1643625958643177No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3710.12343830580093494No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3560.11844753872003459No Hit
GCGTGACAGCCACTGTAGGACTTTGATCTCAGGGGGACAAGCTGACACAG3090.1028098018665469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)