FastQCFastQC Report
Wed 8 May 2024
EGAF00006128367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300555
Total Bases42.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12130.4035866979421404No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG4810.16003726439420407No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3650.12144199896857481No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3550.11811482091464126No Hit
GCGTGACAGCCACTGTAGGACTTTGATCTCAGGGGGACAAGCTGACACAG3350.11146046480677413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)