FastQCFastQC Report
Wed 8 May 2024
EGAF00006128368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371050
Total Bases51 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT202105.446705295782239TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31490.8486726856218839No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4850.13071014688047433No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4670.12585904864573508No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4590.12370300498585095No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA4340.11696536854871312No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA3790.10214256838700984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)