FastQCFastQC Report
Wed 8 May 2024
EGAF00006128370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288137
Total Bases39.7 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATCTCGTAT139884.854635121487348TruSeq Adapter, Index 18 (97% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21060.7309023138298796No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATATCGTAT6000.20823427744441014TruSeq Adapter, Index 18 (97% over 39bp)
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4560.1582580508577517No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4290.14888750837275325No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC3150.10932299565831533No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3110.10793476714201924No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTACTT3070.10654653862572318No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA3050.10585242436757514No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA3000.10411713872220507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)