FastQCFastQC Report
Wed 8 May 2024
EGAF00006128374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258752
Total Bases34.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATCTCGTAT181126.999752658916646TruSeq Adapter, Index 18 (97% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24040.9290749443482562No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATATCGTAT8000.30917635419243134TruSeq Adapter, Index 18 (97% over 39bp)
GCGTGACAGCCACTGTAGGACTCTGATCTCAGGGGGACAGGCTGACACAG3450.13333230274548602No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3410.13178642097452387No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT2600.10048231511254019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)