FastQCFastQC Report
Wed 8 May 2024
EGAF00006128378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381576
Total Bases52.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT186754.894175734322913TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29930.7843784724406148No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4730.12395957817053482No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATATCGTAT4320.11321466758915655TruSeq Adapter, Index 7 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA4290.11242845461978741No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA4210.11033188670146968No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4150.10875946076273141No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC4070.10666289284441369No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3970.10404218294651656No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC3820.10011111809967084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)