FastQCFastQC Report
Wed 8 May 2024
EGAF00006128380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330589
Total Bases44.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT174165.268172867215788TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29560.8941616327222018No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA4830.14610286488661148No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC4680.14156550883423225No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4460.13491071995740936No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4130.12492853664217503No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATATCGTAT4000.12099616139677968TruSeq Adapter, Index 7 (97% over 36bp)
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA3840.1161563149409085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)