FastQCFastQC Report
Wed 8 May 2024
EGAF00006128382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379500
Total Bases51.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT176904.661396574440053TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32220.8490118577075099No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT6620.17444005270092225TruSeq Adapter, Index 9 (97% over 36bp)
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC5510.14519104084321474No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA5300.13965744400527008No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA4930.12990777338603426No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4340.11436100131752307No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4300.11330698287220027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)