FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379500
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32540.8574440052700922No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC5610.14782608695652175No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4330.11409749670619235No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4260.11225296442687747No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3910.10303030303030303No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA3910.10303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)