FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429390
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT116712.718041873355225TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28850.6718833694310533No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA11860.27620578029297377No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG8350.1944619110831645No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT7140.1662824006148257No Hit
TGTCAAGTGGGAAATGAATGCTCTTGCAAGGCTCAAACTTGTGAACACAT6040.14066466382542678No Hit
CTGAAGTAGATGAACAGCCCTGTCCCAAGGAAGAGCAGGCCCAGCACAAA5810.13530822795127972No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA5760.1341437853699434No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA5150.11993758587764036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)