FastQCFastQC Report
Wed 8 May 2024
EGAF00006128385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429390
Total Bases59.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29270.6816646871142784No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA11660.2715480099676285No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG7770.18095437713966323No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT6850.15952863364307507No Hit
TGTCAAGTGGGAAATGAATGCTCTTGCAAGGCTCAAACTTGTGAACACAT6150.14322643750436667No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA5750.13391089685367616No Hit
CTGAAGTAGATGAACAGCCCTGTCCCAAGGAAGAGCAGGCCCAGCACAAA5620.13088334614220173No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4970.11574559258482964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)