FastQCFastQC Report
Wed 8 May 2024
EGAF00006128386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333696
Total Bases48.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT121583.643435941695435TruSeq Adapter, Index 2 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA13970.4186445147679325No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA11330.3395305907172996No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7700.23074894514767932No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG5270.15792817414652857No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4960.14863828154967396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)