FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326428
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT119553.6623696496624065TruSeq Adapter, Index 11 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10160.31124780962417437No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA6450.19759334370826032No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA6200.18993468697538202No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4650.1424510152315365No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4230.12958447192030095No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT4190.12835908684304043TruSeq Adapter, Index 11 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)