FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431917
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT123912.8688382258628393TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20780.4811109541879574No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT6310.1460928835864298No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA5910.13683184500725834No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCTTTCTA4540.10511278787359607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)