FastQCFastQC Report
Wed 8 May 2024
EGAF00006128394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346577
Total Bases48.1 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATCTCGTAT123983.577271428859965TruSeq Adapter, Index 18 (97% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22560.6509375982826326No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATATCGTAT6190.17860388889049186TruSeq Adapter, Index 18 (97% over 39bp)
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT5090.1468649102508245No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5020.14484515706466383No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC4920.1419597953701486No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA4500.12984127625318473No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4060.11714568479731778No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA3730.10762399120541756No Hit
GGGGTTCATGCCTGAGATTCCCTTCCTGGAGGAGCCTCAGTGTTACAATG3550.10243034015529016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)