FastQCFastQC Report
Wed 8 May 2024
EGAF00006128399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311936
Total Bases43.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18320.5872999589659417No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA4870.15612176856791138No Hit
CGGATCCTCCTCCAGCTCCTGCTTGGAGGTCTCCAGAATAGGCTGGAGGC4010.12855201066885516No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3970.1272696963479688No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT3800.12181986048420189No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC3740.11989638900287239No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACATCAGCCCCTCTA3720.11925523184242921No Hit
GCATTTCCTTGCTTGCTCACTTCAGTCCCTCTAGCCTTCTTTCTGTCCCT3520.11284366023799754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)