FastQCFastQC Report
Wed 8 May 2024
EGAF00006128400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352874
Total Bases49 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT171634.863775738648923TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27330.7744974126742122No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG6740.1910030209083129No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4920.13942653751764086No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4450.12610733576290686No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT4260.12072297760673781No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATATCGTAT4050.11477184490781413TruSeq Adapter, Index 7 (97% over 36bp)
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACGGTGGACACG3920.11108781037990897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)